Engineered Heart Tissues formed with Cardiac Progenitors and Differentiated Cardiomyocytes Exhibit Similar Physiologic Properties at Differentiation-Matched Timepoints [dataset]
Description
This dataset contains supporting data for the publication "Engineered Heart Tissues formed with Cardiac Progenitors and Differentiated Cardiomyocytes Exhibit Similar Physiologic Properties at Differentiation-Matched Timepoints". This study demonstrated that induced pluripotent stem cell-derived cardiovascular progenitors (iPSC-CVP) can be used to form functional engineered heart tissues with similar electrophysiological properties to tissues formed from fully differentiated induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM), while also being more amenable to enzymatic dissociation and mechanical manipulation.
Files
Steps to reproduce
The physiology data were generated with high-speed microscopy. The molecular data were generated on ACEA NovoCyte flow cytometer and Beckman Coulter MoFlo cell sorter. Other data were generated via ImageJ analysis of histological images, and quantitative analysis. Refer to the Methods section of the publication. Physiology data were analyzed in Matlab software. Matlab code is available at huebschlab.wustl.edu. Molecular data were analyzed using the Seurat v.5.0 package according to provided tutorials (https://satijalab.org/seurat/).
Institutions
- Washington University in St. Louis
Categories
Funders
- Additional VenturesGrant ID: Additional Ventures Cures Collaborative
- Sarnoff Cardiovascular Research FoundationUnited States
Additional Metadata for Digital Commons Data@Becker
| Keywords | Induced pluripotent stem cell, iPSC, iPSC-CVP, iPSC-CM, Engineered heart tissue, Electrophysiology, Regenerative medicine, Bioprinting, Calcium imaging, Cardiac maturation, Congenital heart disease |