Washington University School of Medicine in St. Louis Showcase

This dataset contains quantitative summary data supporting the publication “Direct activation of SARM1 by dsDNA is not supported by biochemical and cellular evidence.” The data include biochemical and biophysical measurements of purified human SARM1 activity and nucleic acid interactions, including NMR, LC-MS/MS, HPLC, fluorescence-based enzymatic assays, electrophoretic mobility shift assays, mass photometry, size-exclusion chromatography, microscale thermophoresis, and electron microscopy; cell-based measurements of NAD+ metabolism, ATP content, and viability in HEK293T cell lines; and axon degeneration and fluorescence imaging assays in primary mouse dorsal root ganglion neurons. These data were used to generate the quantitative results presented in Figures 1–4 and associated supplemental figures and support the study’s evaluation of whether double-stranded DNA directly activates SARM1, the role of PARP-dependent NAD+ depletion in dsDNA-induced cellular stress, and the functional consequences of the SARM1 3KE mutant. The experimental sources include purified recombinant human SARM1 proteins, human HEK293T cell lines, and primary dorsal root ganglion neurons from wild-type and Sarm1-knockout mice.

In this study, we present amyloid-β-specific engineered CD4+ CAR-T as an approach to directly target sites of pathology. The data is presented in a single spreadsheet file containing all raw data for main and supplementary figures.

In this study we showed that local lentiviral delivery of miR-181a/b-1 enhanced ulnar fracture repair in male and female mice, and in vitro osteogenesis in human cartilage precursor cells. Mechanistically, miR-181a/b-1 directly targeted and suppressed pyruvate dehydrogenase kinase 4 (PDK4), thereby increasing mitochondrial respiration. Consistent with this mechanism, treatment with the PDK4 inhibitor diisopropylamine dichloroacetate (DADA) increased osteogenesis and mitochondrial metabolism in vitro and enhanced endochondral fracture repair in vivo, recapitulating the effects of miR-181a/b-1. This dataset comprises 35 .csv files, providing data related to Figures 2, 3, 4, 5, 6, S9 and S12.

This dataset includes raw z-stack files from the Nikon microscope as well as deleted-slices versions of some of them, and Imaris files for which the original microscope z-stacks are not available. These correspond to the images and movies in the manuscript "Longitudinal single-axon-resolution imaging of peripheral nerve injury response in mice using an optical window implant". The experimental conditions and other information about the images is in the manuscript and the file metadata.

This collection brings together the Knight Alzheimer Disease Research Center (ADRC) Data Freeze datasets published through Digital Commons Data@Becker. Each Data Freeze represents a distinct snapshot of the ADRC research data available at a specific point in time. Individual Data Freeze datasets are assigned separate DOIs to preserve versioned releases, while this collection provides a single entry point for browsing the complete series.

This dataset provides supporting data for the manuscript titled "Chronic oral cannabidiol delays seizure onset and reduces seizure burden in a mouse model of CLN2 disease" and is deposited to comply with PLOS One data availability requirements. The data shows that chronic treatment with cannabidiol confers significant anti-seizure benefit to the mouse model of CLN2 disease, and that it does not appear to do so by altering the inflammatory and neuroimmune markers traditionally used to track CLN2 disease progression. There are four files corresponding to Figures 1 to 3 and Table S1.

This study uses a community-based qualitative research approach and a critical policy analysis framework to examine how a postdoctoral health benefits policy is interpreted, implemented, and experienced at a private research-intensive university. Data were collected through role specific focus groups with 20 participants, including postdoctoral researchers, faculty advisors, and administrative staff, and analyzed using thematic analysis. Themes coded for in the study are defined. This study was determined by Washington University Human Research Protection Office to be a Quality Assurance/Quality Improvement study that is not subject to IRB oversight.

Using mouse models of alphavirus-induced arthritis, we investigated how antibiotic-induced gut dysbiosis shapes inflammatory responses in the joint. We found that depletion of microbiota-derived short chain fatty acids (SCFAs) activated pathogenic immune cells in the gut that drove more severe arthritis, while restoring SCFAs or SCFA-producing bacteria attenuated disease. Data were generated using flow cytometry, fluorescent activated cell sorting, RNA sequencing, qRT-PCR, in situ hybridization, H&E staining, Toulidine blue staining, TRAP staining, focus forming assay, ELISA, liquid chromatography-mass spectrometry, virus and bacterial cultures, bacterial engineering, and in vivo characterization. There are 10 main Figures and 7 Supplementary Figures. Data were obtained using gnotobiotic and specific pathogen free C57BL6/J wild type mice, as well as specific pathogen free Rag1 KO (Rag1tm1Mom, 034159); Tcrbd KO (Tcrbtm1Mom Tcrdtm1Mom, 002122); µMT (Ighmtm1Cgn, 002288); Myd88 KO (MyD88tm1.1Defr, 009088); Il1r KO (Il1r1tm1Imx, 003245); Tlr7 KO (Tlr7tm1Flv, 008380); Tlr9 KO (Tlr9M7Btlr, 34329-Jax); Tlr2 KO (Tlr2tm1Kir, 004650); Tlr4 KO (Tlr4tm1.2Karp, 029015); Pou2f3 KO (Pou2f3em1Cbwi, 037040); CD45.1 OT-II Rag1 KO; Myd88f/f (008888) x Villin-Cre (004586); and Myd88f/f (008888) x Ccr2-Cre-ERT2 (035229) mice. Vero CCL-81 cells were also used in this project.

The Haiti Cardiovascular Disease (HCVD) Cohort is a longitudinal, community-based cohort study designed to investigate cardiovascular disease (CVD) and related risk factors among adults living in Port-au-Prince, Haiti. This dataset contains enrollment data collected from March 1, 2019 through August 31, 2021 and was used for a cross-sectional analysis examining the association between depressive symptoms and prevalent cardiovascular disease among urban Haitian adults. The dataset includes demographic and socioeconomic characteristics (age, sex, education, marital status, occupation, and household income); health behaviors (smoking, alcohol use, and physical activity); psychosocial measures including the Patient Health Questionnaire-9 (PHQ-9) and Perceived Stress Scale-4 (PSS-4); household food insecurity indicators; anthropometric measurements (height and weight); blood pressure measurements; antihypertensive medication use; and adjudicated cardiovascular disease outcomes. Cardiovascular disease outcomes include angina, myocardial infarction, heart failure, stroke, and transient ischemic attack and were classified using epidemiologic definitions and adjudication procedures developed for the HCVD Cohort. The dataset was used to evaluate the association between depressive symptoms and prevalent cardiovascular disease while accounting for demographic, socioeconomic, behavioral, and psychosocial factors. The data support research on cardiovascular health, mental health, social determinants of health, health disparities, and chronic disease epidemiology in low-resource settings. Files included in this deposit are: (1) a CSV file containing the analytic dataset; (2) a comprehensive data dictionary documenting variable definitions, data types, units of measurement, allowable values, coding schemes, and variable derivations; and (3) an R Markdown (.Rmd) file containing the reproducible analytical workflow used to clean the data, derive study variables, perform statistical analyses, and generate the tables, figures, and results reported in the associated manuscript, “Depressive Symptoms and Association with Prevalent Cardiovascular Disease: A Cross-Sectional Analysis in Port-au-Prince, Haiti.”

In this work, we characterized the immunogenicity and efficacy of a Matrix-M®–adjuvanted nanoparticle protein vaccine containing recombinant H5 HA of A/American wigeon/South Carolina/22/000345-001/2021 virus (clade 2.3.4.4b, H5-MNP). Influenza A virus (H5N1) animal challenge model involves BL/6 mice and Madin-Darby Canine Kidney epithelial (MDCK) cells. Data types include Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) imputed values from Quant Studio, Focus-forming assay (FFA) values from CTL Biospot, histological images, and weight loss data.