Washington University School of Medicine in St. Louis Showcase

This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "An intranasal adenoviral-vectored vaccine protects against highly pathogenic avian influenza H5N1 in naïve and antigen-experienced animals" intended for publishing in Cell Reports Medicine. The dataset includes antibody and T-cell responses in naïve and vaccine-immunized mice as well as antibody responses in hamsters after intranasal ChAd-vectored H5 vaccination, along with viral titers following H5N1 challenge.

This phase 3 double-blind, placebo-controlled randomized clinical trial, Starting a Testosterone and Exercise Program After Hip Injury (STEP-HI), was conducted at 8 US sites between December 2018 and August 2023. Participants were women aged 65 years or older with a recent surgical repair of a nonpathologic femur fracture, met objective criteria for mobility impairment, and were community dwelling after discharge from rehabilitation. During the first 14 months of the trial, participants were randomly assigned to 1 of 3 treatment groups: exercise plus topical testosterone gel, exercise plus placebo gel, or enhanced usual care. Statistical analysis, using a modified intention-to-treat approach, was performed from November 2023 to November 2024.

This dataset contains all raw data underlying the main and supplementary figures of the manuscript “Identification of Immunodominant T Cell Epitopes in the SARS-CoV-2 Spike Protein in Syrian Hamsters” published in The Journal of Immunology. It includes measurements of T cell responses in Syrian hamsters immunized with a replication-defective ChAd-SARS-CoV-2-S vaccine encoding a pre-fusion–stabilized Wuhan-1 spike protein. The dataset also provides comparative T cell–response data following in vivo CD4 or CD8 depletion. In addition, it identifies key CD4 and CD8 epitopes within the most immunogenic regions of the spike protein.

This dataset contains supporting data for the publication "Engineered Heart Tissues formed with Cardiac Progenitors and Differentiated Cardiomyocytes Exhibit Similar Physiologic Properties at Differentiation-Matched Timepoints". This study demonstrated that induced pluripotent stem cell-derived cardiovascular progenitors (iPSC-CVP) can be used to form functional engineered heart tissues with similar electrophysiological properties to tissues formed from fully differentiated induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM), while also being more amenable to enzymatic dissociation and mechanical manipulation.

This dataset includes raw data for seven main and eight supplementary figures from the manuscript "MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after WNV infection." In this in vivo mouse study, loss of type I IFN signaling reveals that IL-1R/MyD88 pathways in hematopoietic myeloid cells drive WNV pathogenesis, causing gut barrier injury, liver damage, cytokine elevation, and brain inflammation.

Excel file containing Tables S1–S3, each provided in a separate tab. Tables S1 and S2 present differential gene expression analyses from bulk RNA sequencing of mouse hearts treated with isoproterenol (ISO) or lipopolysaccharide (LPS). Related RNA-seq data are available in GEO accession GSE307900. Table S3 presents differential chromatin accessibility analyses from bulk ATAC sequencing of bone marrow–derived hematopoietic stem and progenitor cells (HSPCs) from mice treated with ISO or LPS, focusing on loci associated with genes implicated in clonal hematopoiesis. Related ATAC-seq data are available in GEO accession GSE305274.

Using CRISPR-Cas9 screen, we identified LRP4 as an entry receptor for Yellow Fever virus. By screening the whole LDLR family members, we further identified LRP1, and VLDLR also act as receptors for Yellow Fever virus. Data were generated by NGS, flow cytometry, qRT-PCR, H&E staining, Bio-Layer Interferometry. There are 5 main Figures and 10 Extended Data Figures. Data were obtained from multiple cell lines including 293T, Vero, HepG2, HAP1, K562 and Raji cells. C57BL/6J mice, AG129 mice and hFGR mice were also used in this project.

This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "LRP8 is an entry receptor for tick-borne encephalitis viruses" published in PNAS. In this study, we expressed 13 different family members on the surface of cells and demonstrated that LRP8 promotes infection of tick-borne encephalitis viruses (TBEV). Validation studies confirmed direct binding of TBEV and LRP8, and a key role for LRP8 in neuronal cell infection. Our findings also enabled the generation of an inhibitory LRP8-Fc decoy molecule that blocks TBEV infection.

In the accompanying publication, we reported on the production and characterization of the broadest K. pneumoniae capsule bioconjugate vaccine to date. We tested this vaccine for its immunogenicity, functionality, efficacy, and antibody durability against a variety of K. pneumoniae isolates in a murine bacteremia model. We also established an immunocompromised murine model of bacteremia to better recapitulate human infection and tested our vaccine’s efficacy in this background. The data included in the metadata set includes ELISAs, serum bactericidal assay, opsonophagocytosis assay, and survival curves. All data was generated in mice. GraphPad Prism is needed to open the data files. To view the data, use the free viewer mode of the software.

Bourbon virus (BRBV) is an emerging tick-borne pathogen that causes severe, often fatal illness marked by cytopenia and thrombocytopenia. With no approved therapies or vaccines, treatment options remain limited. Using a preclinical mouse model, we evaluated molnupiravir, a broad-spectrum antiviral approved in the U.S. Molnupiravir reduced viral replication in cells and tissues, improved survival, and alleviated disease signs. These results highlight its potential as a therapeutic candidate for BRBV infection.