Dataset for 'Intranasal boosting induces variant-specific responses in SARS-CoV-2 vaccine-imprinted mice'

Published: 22 May 2026| Version 1 | DOI: 10.17632/9drz7yrmny.1
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Description

This dataset contains the raw data supporting main Figures 1-5 and Extended Data Figures 1-10 used in the manuscript "Intranasal boosting induces variant-specific responses in SARS-CoV-2 vaccine-imprinted mice" submitted to Nature Communications. The dataset includes antibody titers in the serum and bronchoalveolar lavage fluid of mice immunized with SARS-CoV-2 mRNA or chimpanzee adenoviral-vectored vaccines at various regimens. The dataset also includes pseudovirus neutralization results and flow cytometry analysis of vaccinated mice. Vaccinations were conducted in wild-type C57BL/6J mice or animals expressing human ACE2 (K18-hACE2 mice). Neutralization assays were performed using Vero cells overexpressing TMPRSS2.

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Steps to reproduce

Refer to the "Methods" section of the paper, including "Cells and viruses", "Chimpanzee adenovirus vectors", "Mouse experiments", "Viral antigens", "Spike-binding assay", "Antibody depletion assay", "Pseudovirus neutralization assay", "Spike-specific B cell staining".

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Categories

Immunology, Infectious Disease, Vaccine

Funders

Additional Metadata for Digital Commons Data@Becker

KeywordsSARS-CoV-2, vaccine, mRNA, ChAd, antibody, antigen-specific, intranasal

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