Dataset for "Projection-TAGs Enable Multiplex Projection Tracing and Multi-modal Profiling of Projection Neurons"
Description
Projection-TAGs is a retrograde AAV platform that allows multiplex tagging of projection neurons using RNA barcodes. The dataset contains the sequencing data and analysis meta data, as well as R scripts, that are required to reproduce the snRNA-seq analysis and combinatorial snRNA-seq and snATAC-seq analysis related to Fig 2 (a multi-modal single-cell atlas of mouse cortex), Fig 3 (Projection-TAGs revealed axonal collaterals and complex wiring diagram), Fig 4 (Genomic regulatory networks of cortical cell types using Projection-TAGs), and Fig 5 (Projection-TAGs enable the detection of projection neurons acutely activated by visceral pain) of the manuscript.
Files
Steps to reproduce
The experimental procedures are available at protocol.io (https://doi.org/10.17504/protocols.io.x54v9r94qv3e/v1). Also refer to the Methods section of the Nature Communications publication.
Institutions
- Washington University in St. LouisMO, Saint Louis
Categories
Funders
Additional Metadata for Digital Commons Data@Becker
| Keywords | Mouse Cortex, Single-cell RNA-seq, Single-cell ATAC-seq, Projection Tracing |
| Revision History | Version 2: major update; adding the "Codes" folder for the dataset; adding contributors Fang Liu and Hannah Hahm; revising description of data; providing links to the Nature Communication publication, GEO record, protocols.io record; adding funder "National Cancer Institute"; other minor revision in the Master-README file. Version 3: add ROR ID; add ORCID. |