Dataset for "MRN-CtIP, EXO1, and DNA2-WRN/BLM act bidirectionally to process DNA gaps in PARPi-treated cells without strand cleavage"
Description
This dataset supports the publication titled “MRN-CtIP, EXO1, and DNA2-WRN/BLM act bidirectionally to process DNA gaps in PARPi-treated cells without strand cleavage,” published in Genes & Development. It contains data from a range of experimental approaches, including single-molecule DNA fiber analysis, immunofluorescence, flow cytometry, electron microscopy, metaphase spread assays, and biochemical analyses. All cellular assays were conducted in human cell lines, while biochemical experiments used purified proteins from both human and yeast sources. The complete dataset is approximately 88 GB in size and includes 66,960 files organized into two main folders. The Figures folder contains 44,103 files across 1,651 subfolders and is 69.4 GB in size. The Supplemental Figures folder contains 22,805 files across 880 subfolders and is 18.7 GB.
Files
Steps to reproduce
For the single-molecule DNA fiber experiments, all the data were collected using our previously published protocol (https://doi.org/10.1016/bs.mie.2017.03.019). The individual tracts were measured using the “multi-point” tool of ImageJ (NIH, https://imagej.nih.gov/ij/). The electron microscopy experiments were performed following our previously published protocol (https://doi.org/10.1007/978-1-0716-2063-2_6). All the remaining protocols are described in details in the README files.
Institutions
- Washington University in St. Louis
- Istituto di Ricerca in Biomedicina
Categories
Funders
- Barnes-Jewish HospitalUnited StatesGrant ID: The Alvin J. Siteman Cancer Center Siteman Investment Program
Additional Metadata for Digital Commons Data@Becker
| Keywords | DNA replication, BRCA, DNA Breaks, DNA Repair, BRCA-mutant tumors, Nucleases, Genome Stability, PARP Inhibitors, DNA, single-stranded DNA |