Dataset for 'Direct activation of SARM1 by dsDNA is not supported by biochemical and cellular evidence'
Description
This dataset contains quantitative summary data supporting the publication “Direct activation of SARM1 by dsDNA is not supported by biochemical and cellular evidence.” The data include biochemical and biophysical measurements of purified human SARM1 activity and nucleic acid interactions, including NMR, LC-MS/MS, HPLC, fluorescence-based enzymatic assays, electrophoretic mobility shift assays, mass photometry, size-exclusion chromatography, microscale thermophoresis, and electron microscopy; cell-based measurements of NAD+ metabolism, ATP content, and viability in HEK293T cell lines; and axon degeneration and fluorescence imaging assays in primary mouse dorsal root ganglion neurons. These data were used to generate the quantitative results presented in Figures 1–4 and associated supplemental figures and support the study’s evaluation of whether double-stranded DNA directly activates SARM1, the role of PARP-dependent NAD+ depletion in dsDNA-induced cellular stress, and the functional consequences of the SARM1 3KE mutant. The experimental sources include purified recombinant human SARM1 proteins, human HEK293T cell lines, and primary dorsal root ganglion neurons from wild-type and Sarm1-knockout mice.
Files
Steps to reproduce
Complete methods available at https://doi.org/10.1073/pnas.2620112123
Institutions
- Washington University in St. LouisMissouri, St Louis
- Griffith UniversityQueensland, Brisbane
- The University of QueenslandQueensland, Brisbane
- National Institute of Biological Sciences, BeijingBeijing, Beijing
- Peking UniversityBeijing, Beijing
- Chinese University of Hong KongHong Kong
- Marche Polytechnic UniversityThe Marches, Ancona
Categories
Funders
- National Institutes of HealthUnited States Department of Health and Human ServicesMaryland, BethesdaGrant ID: NS087632
- National Institutes of HealthUnited States Department of Health and Human ServicesMaryland, BethesdaGrant ID: NS065053
- National Health and Medical Research CouncilDepartment of Health and Aged CareAustralian Capital Territory, CanberraGrant ID: 1196590
- National Health and Medical Research CouncilDepartment of Health and Aged CareAustralian Capital Territory, CanberraGrant ID: 2042089
- National Health and Medical Research CouncilDepartment of Health and Aged CareAustralian Capital Territory, CanberraGrant ID: 2025931
- Ministry of Science and Technology of the People's Republic of ChinaState Council of the People's Republic of ChinaBeijing, BeijingGrant ID: Institutional grant (to X. Wang)
- Beijing Municipal Science and Technology CommissionMinistry of Science and Technology of the People's Republic of ChinaBeijing, BeijingGrant ID: Institutional grant (to X. Wang)
- National Facility for Structural Biology, Fondazione Human TechnopoleGrant ID: Call for Access 25-SB-ROUND-1, Project ID 2160736 (to G. Orsomando)
Additional Metadata for Digital Commons Data@Becker
| Keywords | SARM1, Nadase, Axon Degeneration, DNA, Neurodegeneration |