Supplemental data: The sphingosine-1-phosphate pathway is differentially activated in human gestational tissues

Published: 1 April 2026| Version 1 | DOI: 10.17632/nxyvc4hjfc.1
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Using qPCR and targeted LC–MS/MS, we profiled S1P metabolic enzymes, receptors, and metabolites in human gestational tissues across pregnancy. These findings reveal tissue-specific regulation of S1P metabolism and signaling in human gestational tissues, suggesting a therapeutic role of S1P in modulating myometrial contractility. The collection of this data was conducted with the approval of the Washington University in St. Louis Institutional Review Board (ID# 202205099).

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Myometrium, decidua parietalis, and chorioamnion were collected from women undergoing cesarean sections at term (≥37 weeks’ gestation) without labor (TNL, n=8), term with labor (TL, n=5), and preterm (<37 weeks’ gestation) without labor (PTNL, n=6). Messenger RNA (mRNA) expression of S1P metabolic enzymes and receptors was assessed using quantitative polymerase chain reaction, while sphingolipids were quantified using targeted liquid chromatography-tandem mass spectrometry. Full methodology is outlined in the manuscript.

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Reproductive Medicine

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