Dataset for 'Multiple LDLR family members act as entry receptors for Yellow Fever virus'
Published: 10 October 2025| Version 1 | DOI: 10.17632/xnpzsx3729.1
Contributors:
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Using CRISPR-Cas9 screen, we identified LRP4 as an entry receptor for Yellow Fever virus. By screening the whole LDLR family members, we further identified LRP1, and VLDLR also act as receptors for Yellow Fever virus. Data were generated by NGS, flow cytometry, qRT-PCR, H&E staining, Bio-Layer Interferometry. There are 5 main Figures and 10 Extended Data Figures. Data were obtained from multiple cell lines including 293T, Vero, HepG2, HAP1, K562 and Raji cells. C57BL/6J mice, AG129 mice and hFGR mice were also used in this project.
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Steps to reproduce
Refer to the Methods section of the publication for details about CRISPR-Cas9 screen, Lentivirus, flow cytometry, protein purification, i.p. infection of mice, i.c. infection of mice, H&E staining.
Institutions
- University of Wisconsin Madison
- Vanderbilt University Medical Center
- Washington University in St. Louis
Categories
Microbiology, Virology, Infectious Disease
Funders
Additional Metadata for Digital Commons Data@Becker
| Keywords | CRISPR-Cas9, Receptor, LRP1, LRP4, VLDLR, Yellow Fever virus |