Dataset for 'An intranasal adenoviral vectored vaccine protects against highly pathogenic avian influenza H5N1 in naïve and antigen-experienced animals'
Description
This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "An intranasal adenoviral-vectored vaccine protects against highly pathogenic avian influenza H5N1 in naïve and antigen-experienced animals" intended for publishing in Cell Reports Medicine. The dataset includes antibody and T-cell responses in naïve and vaccine-immunized mice as well as antibody responses in hamsters after intranasal ChAd-vectored H5 vaccination, along with viral titers following H5N1 challenge.
Files
Steps to reproduce
Refer to the "Materials and Methods" section of the paper, including "Construction of chimpanzee adenoviral vectors", "Generation of recombinant influenza virus HA proteins", "HA multiplex binding assays", "Antibody depletion assay", "Virus neutralization assay", "Mouse and hamster immunization and challenge experiments", "Virological analysis (RT-qPCR, FFA assays)", "Lung histology and viral RNA in situ hybridization", "T cell profiling and depletion and cell analysis".
Institutions
- Washington University in St. LouisMO, Saint Louis
- La Jolla Institute for Allergy and ImmunologyCA, La Jolla
Categories
Funders
Additional Metadata for Digital Commons Data@Becker
| Keywords | Adenoviral-vectored vaccine, Intranasal vaccination, Avian influenza H5N1 virus, Hemagglutinin (HA), Mucosal immunity, Neutralizing antibodies, T cell responses, Pre-existing immunity, Quadrivalent influenza vaccine (QIV) |