Proteomics data for 'How Does Tendon Region, Donor, and the Presence of Disease Affect Protein Composition of the Achilles Tendon?'
Description
Proteomics data from Achilles tendon specimens from human patients undergoing surgery for lower extremity amputation (n=10; 5 with diabetes, 5 non-diabetes controls) or Achilles tendon debridement/repair for Achilles tendinopathy (n=10). The collection of this data was conducted with the approval of the Washington University in St. Louis Institutional Review Board (ID# 201505110 and 202111069). Participants provided informed consent for the sharing of de-identified data.
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Non-tendinopathic tendon specimens from non-diabetic (n=5) and diabetic (n=5) individuals were collected from individuals undergoing lower extremity amputation. Tendinopathic tendons (n=10) specimens were collected from individuals undergoing surgery for Achilles tendinopathy. In all cases, individuals in the tendinopathic group were having surgery for insertional Achilles tendinopathy. All participants needed to be at least 18 years of age. Exclusion criteria included pregnancy, previous amputations that had removed the Achilles tendon tissue, infection extending into the tendon, macroscopic tendon injury/tendon thickening on visual inspection (for non-tendinopathic groups), and certain infectious diseases (history of human immunodeficiency virus or hepatitis C; current methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococcus, or COVID-19 infection). Additional exclusion criteria for the tendinopathy group were history of collagen disorders (e.g. Ehlers-Danlos syndrome), rheumatoid arthritis, or lupus. Participants were enrolled/specimens were collected between April 2019 and August 2023. Due to the precious nature of these samples, matching was not able to be performed between cases and controls. The Achilles tendons from participants undergoing lower extremity amputation (noted with MB identifier) were dissected and collected by a member of the study team immediately following surgical removal of the affected limb. Achilles tendon specimens from participants with tendinopathy (noted with AT identifier) were tissue sections debrided by the surgeon as part of routine surgical procedure during Achilles tendon debridement with repair surgery and passed to a member of the study team. All specimens were wrapped in phosphate-buffered saline soaked gauze, kept on ice bath during transport, and stored at -80°C until analysis. For tendon specimens from lower extremity amputation, an approximately 1 mm transection was made from the distal aspect of the tendon. This section was then divided into thirds to yield 2 periphery and 1 central “core” specimen. For regional analysis, the peripheral and core specimens were compared. The core specimen was used for all remaining analyses. A second ~1 mm transection from the distal aspect of the remaining surgical tendon specimen was made and fixed for histology. Full methods are included in the publication, 'How Does Tendon Region, Donor, and the Presence of Disease Affect Protein Composition of the Achilles Tendon?'.
Institutions
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Funders
- National Institute of Arthritis and Musculoskeletal and Skin DiseasesUnited StatesGrant ID: P30AR074992
- Lottie Caroline Hardy Charitable TrustUnited States
- Washington University in St. LouisUnited StatesGrant ID: Program in Physical Therapy internal funding
- Washington University in St. LouisUnited StatesGrant ID: Musculoskeletal Research Center Pilot & Feasibility Award Year 15
Additional Metadata for Digital Commons Data@Becker
| Keywords | Achilles, Tendon, Human, Proteome, Tendinopathy, Type 2 Diabetes Mellitus |