Real-Time Photothrombosis (RT-PT) Enables Spatially Versatile Focal Ischemia and Continuous Electrophysiological Monitoring in Freely Behaving Mice [dataset]
Description
Poststroke sleep disruption is a major determinant of recovery, yet how lesion location shapes hyper-acute and acute changes in sleep architecture remains poorly understood. To address this gap, we developed real-time photothrombosis (RT-PT) in a mouse model that enables spatially targeted focal ischemia with continuous EEG/EMG monitoring from the moment of stroke induction without the confounding effects of anesthesia. This dataset provides real-time electrophysiological recordings (EEG/EMG) from 18 mice undergoing focal ischemia via RT-PT. By delivering laser light through implanted optical fibers, we induced titratable ischemic strokes in the forelimb somatosensory cortex (SSC), intralaminar thalamus (IT), or CA1 region of the hippocampus (CA1) while animals remained awake and freely behaving. The dataset captures neural dynamics across the hyper-acute (0–24 hr) and acute (1–4 days) phases of stroke, comprising 18 raw EDF recordings and 270 manually corrected AccuSleep scoring files. The shared dataset is organized into a hierarchical structure indexed by target infarct region and Mouse ID. Root Directory: RT-PT Dataset (AccuSleep Files: Divided into target region subfolders (SSC, IT, and CA1). Each folder contains the raw, continuous 5-day EDF recording for each mouse, alongside 15 AccuSleep files (signal, label, and calibration) split into 24-hour intervals.; Codes: Contains 13 scripts and notebooks organized into three functional subfolders: Vigilance State\, \Fragmentation\, and \Spectra\, with each subfolder housing the respective code files for that analysis.; Analysis (Master Directory): Contains the consolidated "Sleep Macrostructure Analysis.csv" master spreadsheet, which aggregates all vigilance and fragmentation values from the individual AccuSleep labels. This directory also includes individual infarct images and composite PDF figures for histological validation.; GraphPad Prism Files: Contains four files that directly visualize the data from the master CSV, illustrating both raw and normalized longitudinal trends.; Additional Information: Supplemental materials, including the conference poster (.pptx and .pdf) detailing the RT-PT methodology and the guideline for AccuSleep scoring and EDF analysis with the codes provided. Additional information is in the Manuscript.docx file.
Files
Steps to reproduce
Refer to the following sections of Manuscript.docx file for more information: Experimental Design, Methods, Directory Structure, Usage Notes.
Institutions
- Washington University in St. LouisMissouri, St Louis
Categories
Funders
- National Institute of Neurological Disorders and StrokeNational Institutes of HealthBethesdaGrant ID: K08NS109292-01A1
Additional Metadata for Digital Commons Data@Becker
| Keywords | Photothrombosis, Stroke model, Focal ischemia, Real-time EEG, Poststroke sleep, Sleep architecture, Sleep fragmentation, Somatosensory cortex, Thalamus, Hippocampus |