Dataset for 'Fc-engineered antibodies enhance protection against SARS-CoV-2 lung infection and inflammation'

Description

This dataset contains the raw data supporting main Figures 1-5 and Supplementary Figures 1, 2, 3, 4, 6 used in the manuscript "Fc-engineered antibodies enhance protection against SARS-CoV-2 lung infection and inflammation" submitted to mBio. The dataset includes viral RNA and infectious virus titers of mice treated with control antibodies or S309 monoclonal antibodies given either before or after SARS-CoV-2 infections. The dataset also includes flow cytometry analysis, lung ventilation analysis, lung pathology, RNA sequencing analysis, weight loss measurement, and antibody titers of treated mice. Some in vitro characterization of antibodies was generated using Jurkat cells.

Steps to reproduce

Refer to the "Methods" section of the paper, including "Cells", "Viruses", "FcγR binding and activation", "Fc effector functions", "MAb preparation", "ELISA", "Focus reduction neutralization assay", "Mouse experiments", "Measurement of viral RNA", "Plaque assay for infectious virus", "Blockade of monocyte recruitment", "Neutrophil depletion", "Respiratory mechanics", "Histology", "Flow cytometry analysis", "Sorting of myeloid cells for bulk RNA sequencing", "RNA sequencing", and "RNA sequencing analysis".

Institutions

• Washington University in St. Louis

  MO, Saint Louis
• Ragon Institute

  MA, Charlestown
• Universitatsklinikum Regensburg

  Bayern, Regensburg
• Vir Biotechnology (Switzerland)

  Ticino, Bellinzona

Categories

Funders

• National Institute of Allergy and Infectious Diseases

  National Institutes of Health

  United States

  Grant ID: R01AI157155
• National Institute of Allergy and Infectious Diseases

  National Institutes of Health

  United States

  Grant ID: U19AI181103

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