Washington University School of Medicine in St. Louis Showcase

This dataset contains supporting data for the publication "Engineered Heart Tissues formed with Cardiac Progenitors and Differentiated Cardiomyocytes Exhibit Similar Physiologic Properties at Differentiation-Matched Timepoints". This study demonstrated that induced pluripotent stem cell-derived cardiovascular progenitors (iPSC-CVP) can be used to form functional engineered heart tissues with similar electrophysiological properties to tissues formed from fully differentiated induced pluripotent stem cell-derived cardiomyocytes (iPSC-CM), while also being more amenable to enzymatic dissociation and mechanical manipulation.

This dataset includes raw data for seven main and eight supplementary figures from the manuscript "MyD88 signaling in myeloid cells induces gastrointestinal tract injury and systemic inflammation after WNV infection." In this in vivo mouse study, loss of type I IFN signaling reveals that IL-1R/MyD88 pathways in hematopoietic myeloid cells drive WNV pathogenesis, causing gut barrier injury, liver damage, cytokine elevation, and brain inflammation.

Excel file containing Tables S1–S3, each provided in a separate tab. Tables S1 and S2 present differential gene expression analyses from bulk RNA sequencing of mouse hearts treated with isoproterenol (ISO) or lipopolysaccharide (LPS). Related RNA-seq data are available in GEO accession GSE307900. Table S3 presents differential chromatin accessibility analyses from bulk ATAC sequencing of bone marrow–derived hematopoietic stem and progenitor cells (HSPCs) from mice treated with ISO or LPS, focusing on loci associated with genes implicated in clonal hematopoiesis. Related ATAC-seq data are available in GEO accession GSE305274.

Using CRISPR-Cas9 screen, we identified LRP4 as an entry receptor for Yellow Fever virus. By screening the whole LDLR family members, we further identified LRP1, and VLDLR also act as receptors for Yellow Fever virus. Data were generated by NGS, flow cytometry, qRT-PCR, H&E staining, Bio-Layer Interferometry. There are 5 main Figures and 10 Extended Data Figures. Data were obtained from multiple cell lines including 293T, Vero, HepG2, HAP1, K562 and Raji cells. C57BL/6J mice, AG129 mice and hFGR mice were also used in this project.

This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "LRP8 is an entry receptor for tick-borne encephalitis viruses" published in PNAS. In this study, we expressed 13 different family members on the surface of cells and demonstrated that LRP8 promotes infection of tick-borne encephalitis viruses (TBEV). Validation studies confirmed direct binding of TBEV and LRP8, and a key role for LRP8 in neuronal cell infection. Our findings also enabled the generation of an inhibitory LRP8-Fc decoy molecule that blocks TBEV infection.

In the accompanying publication, we reported on the production and characterization of the broadest K. pneumoniae capsule bioconjugate vaccine to date. We tested this vaccine for its immunogenicity, functionality, efficacy, and antibody durability against a variety of K. pneumoniae isolates in a murine bacteremia model. We also established an immunocompromised murine model of bacteremia to better recapitulate human infection and tested our vaccine’s efficacy in this background. The data included in the metadata set includes ELISAs, serum bactericidal assay, opsonophagocytosis assay, and survival curves. All data was generated in mice. GraphPad Prism is needed to open the data files. To view the data, use the free viewer mode of the software.

Bourbon virus (BRBV) is an emerging tick-borne pathogen that causes severe, often fatal illness marked by cytopenia and thrombocytopenia. With no approved therapies or vaccines, treatment options remain limited. Using a preclinical mouse model, we evaluated molnupiravir, a broad-spectrum antiviral approved in the U.S. Molnupiravir reduced viral replication in cells and tissues, improved survival, and alleviated disease signs. These results highlight its potential as a therapeutic candidate for BRBV infection.

The purpose of this project is to transform an existing, university-based Long COVID clinic into a broader Long COVID community network in order to expand equitable access to care, improve the patient care experience, and support primary care practitioners in the St. Louis metropolitan area and surrounding rural region. De-identified raw survey response data are shared for patients who explicitly consent to this type of sharing. Similarly, for participants who explicitly consent, de-identified interview transcripts are also shared after redacting potentially unique or identifying information. The files include qualitative data codebooks, REDCap data dictionaries, interview guides and protocols, patient and provider survey, analytic code, administrative codebooks, self report codebook. For survey data, REDCap data dictionaries, surveys, survey administration protocols, a codebook of coded variables are shared. Codebooks contains references and scoring information for relevant survey measures or indicate when survey items were developed or adapted specifically for this study. For each variable in the dataset, the codebook specifies the variable name, variable label/content, response values and labels, and any codes for missing values including numeric values and labels (e.g., 99 = don’t know). For interview data, shared items include interview guides and protocols (including description of interview settings and procedures); general descriptions of interviewers and interviewees (including eligibility criteria, sampling criteria, and recruitment methods); dates interviews were conducted; and codebooks containing code names, labels, and any procedural notes. The collection of this data was conducted with the approval of the Washington University in St. Louis Institutional Review Board (ID# 202401001).

Proteomics data from Achilles tendon specimens from human patients undergoing surgery for lower extremity amputation (n=10; 5 with diabetes, 5 non-diabetes controls) or Achilles tendon debridement/repair for Achilles tendinopathy (n=10). The collection of this data was conducted with the approval of the Washington University in St. Louis Institutional Review Board (ID# 201505110 and 202111069). Participants provided informed consent for the sharing of de-identified data.

This dataset supports the publication titled “MRN-CtIP, EXO1, and DNA2-WRN/BLM act bidirectionally to process DNA gaps in PARPi-treated cells without strand cleavage,” published in Genes & Development. It contains data from a range of experimental approaches, including single-molecule DNA fiber analysis, immunofluorescence, flow cytometry, electron microscopy, metaphase spread assays, and biochemical analyses. All cellular assays were conducted in human cell lines, while biochemical experiments used purified proteins from both human and yeast sources. The complete dataset is approximately 88 GB in size and includes 66,960 files organized into two main folders. The Figures folder contains 44,103 files across 1,651 subfolders and is 69.4 GB in size. The Supplemental Figures folder contains 22,805 files across 880 subfolders and is 18.7 GB.